bsc 1  (ATCC)

Journal: Food Science of Animal Resources

Article Title: The L-Ascorbic Acid Increases Proliferation and Differentiation of Yanbian Cattle Skeletal Muscle Satellite Cells by Activating the Akt/mTOR/P70S6K Signaling Pathway

doi: 10.5851/kosfa.2024.e50

Figure Lengend Snippet: Cell viability was measured using CCK-8 after treatment of BSCs with different concentrations (50, 100, 200, 400, and 800 μM) of AA for 24, 48, and 72 h (A). The percentage of EdU-positive cells was detected by EdU staining under fluorescence microscopy after 48 h treatment of BSCs using different concentrations of AA (B; scale bar=200 μm). The histogram shows the statistics of positive cells after 48 h treatment of BSCs using different concentrations of AA (C). The mRNA expression levels of the gene encoding the transporter protein SLC23A2 after 48 h treatment of BSCs with different concentrations (100, 200, and 400 μM) of AA by real-time quantitative PCR (RT-qPCR). The expression levels of Ki67, proliferating cell nuclear antigen (PCNA), cell cycle protein-dependent kinase 1 (CDK1) and CDK2 proliferation-related genes were measured by RT-qPCR at different concentrations (100, 200, and 400 μM) of AA treated BSCs for 48 h (D). The expression levels of proliferation marker paired box 7 (Pax7) were measured by RT-qPCR and Western blotting after treatment of BSCs with different concentrations of AA for 48 h (E, F). All experiments were repeated ≥3 times and compared using t-test. Data are presented as mean±SEM. a–e Significant differences between treatment groups are indicated by different letters (p<0.05), and the same letter or no letter marked indicates no significant difference between groups (p>0.05).

Article Snippet: BSCs were then immunofluorescently stained with an antibody against Pax7 (mouse anti-Pax7,1:200, Proteintech), a key marker factor for BSCs.

Techniques: CCK-8 Assay, Staining, Fluorescence, Microscopy, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Marker, Western Blot

Journal: Food Science of Animal Resources

Article Title: The L-Ascorbic Acid Increases Proliferation and Differentiation of Yanbian Cattle Skeletal Muscle Satellite Cells by Activating the Akt/mTOR/P70S6K Signaling Pathway

doi: 10.5851/kosfa.2024.e50

Figure Lengend Snippet: Cell viability was measured by CCK-8 after 48 h treatment of BSCs using different concentrations (10, 20, 100, 200, and 400 nM) of rapamycin (A). Detection of EdU positive cell rates under fluorescent microscopy using EdU staining (B; scale bar=200 μm). The bar graph shows the differences between the treatment groups (C). The mRNA expression levels of proliferation-related genes paired box 7 (Pax7), Ki67, and proliferating cell nuclear antigen (PCNA) were detected using real-time quantitative PCR (D). The mTOR pathway was used to detect the level of Pax7 protein expression under the regulation of L-ascorbic acid (AA) using Western blotting (E). a–d Significant differences between treatment groups are indicated by different letters (p<0.05), and the same letter or no letter marked indicates no significant difference between groups (p>0.05).

Article Snippet: BSCs were then immunofluorescently stained with an antibody against Pax7 (mouse anti-Pax7,1:200, Proteintech), a key marker factor for BSCs.

Techniques: CCK-8 Assay, Microscopy, Staining, Expressing, Real-time Polymerase Chain Reaction, Western Blot